Objective To construct a lentivirus vector carrying wheat germ agglutinin(WGA) and evaluate its ability of tracing WGA in the brain of mice with ischemic brain injury. Methods WGA gene was inserted into the lentiviral vector Plvx IRESZs Green1 using genetic engineering methods. 293 T cells were transfected with the vector and 3 packaging plasmids(RPEV,PRRE, and VSVG) to obtain the recombinant lentivirus for infection of human adipose- derived stem cells(h ADSCs). The infected h ADSCs were injected into the ...
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