【摘要】 本试验研究黄芪多糖(APS)对种公鸡不同组织中miR-16表达的影响,结合miR-16预测靶基因的GO和KEGG功能富集性分析,旨在探讨APS对不同组织功能的潜在调控作用。选取1日龄科宝500父母代肉种鸡64只,随机分为对照组和APS组,每组4个重复,每个重复8只鸡。对照组饲喂玉米-豆粕型基础饲粮,APS组在基础饲粮中添加10 g/kg APS,试验期40周。结果表明:1)相比于对照组,APS组可以提高种公鸡的采精量和有效精子数,降低精子畸形率,但差异不显著(P>0.05)。2)miR-16具有组织差异性,APS可显著或极显著上调肝脏、脾脏、十二指肠黏膜、回肠黏膜miR-16的表达(P<0.05或P<0.01),显著降低睾丸miR-16的表达(P<0.05)。3)对miR-16预测靶基因进行GO富集分析,表明预测靶基因在跨膜运输、泛素依赖的蛋白质降解、胞内蛋白运输、糖酵解等物质代谢相关生物过程显著或极显著富集(P<0.05或P<0.01)。KEGG富集分析表明,miR-16靶基因在黏着斑、胰岛素信号通路、糖酵解/糖异生等与机体物质代谢、细胞增殖分化相关通路显著富集(P<0.05),在Toll样受体信号通路、自然杀伤细胞介导的细胞毒性等免疫相关通路也有富集。由此可见,APS上调种公鸡脾脏、肝脏、十二指肠黏膜、回肠黏膜组织的miR-16表达,下调睾丸miR-16表达;miR-16预测靶基因与物质代谢、免疫调控密切相关。APS通过调控种公鸡不同组织miR-16表达,影响机体物质代谢和免疫调控功能。更多还原

【Abstract】 This experiment was conducted to investigate the effects of Astragalus polysaccharides( APS) on miR-16 expression in different tissues of breeder cocks,combining with the functional enrichment analysis of GO and KEGG pathw ay of miR-16 predicted targets,and to explore the potential regulation role of APS in different tissues via miR-16. A total of 64 one-day-old Cobb 500 breeder cocks were randomly divided into control group and APS group with 4 replicates per group and 8 breeder cocks per replicate. Cocks in the control group were fed a corn-soybean meal based diet,and the others in APS group were fed the basal diet supplemented with 10 g / kg APS. The experiment lasted for 40 weeks. The results showed as follow s: 1) compared with the control group,dietary supplemented with APS improved the semen volume and number of effective sperm,decreased the percentage of abnormal sperm( P> 0.05). 2) miR-16 differentially expressed in different tissues; APS ssignificantly increased the miR-16 expression in liver,spleen,duodenum mucosa and ileum mucosa( P< 0.05 or P< 0.01),and dow n-regulated miR-16 expression in testis( P< 0.05). 3) GO enrichment analysis of miR-16 predicted that targets were showed to be enriched for the metabolic functions in transmembrane transport,ubiquitin-dependent protein catabolic process,intracellular protein transport,glycolytic process( P<0.05 or P<0.01). KEGG pathway showed that the target genes were enriched in focal adhesion,insulin signaling pathw ay,glycolysis / gluconeogenesis associated with cell proliferation,differentiation and metabolism( P<0.05). And the target genes were also enriched in toll-like receptors signaling pathways and natural killer cell mediated cytotoxicity related to immune pathw ays. In conclusion,APS up-regulates the expression of miR-16 in liver,spleen,duodenum mucosa,ileum mucosa,while dow n-regulates that in testis of breeder cocks.miR-16 predicted targets collect with metabolism and immune regulation,it showes that APS regulated the miR-16 expression on different tissues of breeder cocks to affect the metabolism and immune regulation functions.更多还原