Highly conservative sequence of salmonid alphavirus E1 gene( 702 bp) was synthesized according to sequences of E1 gene of SAV1,SAV2 and SAV3 published in Gen Bank. It was cloned into prokaryotic expression vector p Cold TF to construct recombinant plasmid. The recombinant plasmid was transformed into E. coli BL21 cells. The expression of recombinant plasmid p Cold TF in E. coli BL21 was induced using IPTG at 1. 0 mmol / L. The target protein of 95 k D was obtained and confirmed by SDS-PAGE and Western blot....
| 
推荐 
点击下列相关研究机构和相关文献作者，可以直接查到这些机构和作者被《中国知识资源总库》收录的其它文献，使您全面了解该机构和该作者的研究动态和历史。
