【摘要】 台湾金线莲和浙江金线莲均为兰科开唇兰属植物,是民间常用珍稀草药金线莲的主要基原植物,体内富含多糖。该研究采用超声提取法Ⅰ、Ⅱ及溶剂提取法提取台湾金线莲和浙江金线莲的多糖,对比多糖含量差异;利用比色法比较两种金线莲多糖对羟自由基清除能力的差异,并以秀丽隐杆线虫为模式生物,探讨两种金线莲多糖对于过氧化氢氧化压力下线虫寿命的影响,综合评价台湾金线莲和浙江金线莲多糖的抗氧化活性,为选择和培育优质基原的金线莲提供理论依据。结果显示:(1)溶剂提取法对金线莲多糖的提取最为有效,浙江金线莲的多糖含量显著高于台湾金线莲;(2)随着浓度的增加,多糖对羟自由基的清除率有升高的趋势,浙江金线莲多糖的羟自由基清除率显著高于台湾金线莲多糖;(3)两种金线莲多糖均可延长线虫寿命,但两者对线虫寿命的影响无显著差异。研究表明,两种金线莲均富含多糖且具有抗氧化活性,但多糖含量及抗氧化活性在两种金线莲间均存在差异。更多还原

【Abstract】 Anoectochilus formosanus and Anoectochilus zhejiangensis are Anoectochilus plants,they are main original plants of Anoectochilus roxburghii that is common folk rare herbs and they are rich in polysaccharide.The study extracted polysaccharide of Anoectochilus formosanus and A.zhejiangensis using ultrasonic extraction Ⅰ,Ⅱ,solvent extraction methods to compare polysaccharide content;we use colorimetry method to compare hydroxyl free radical clearance ability of the two A.roxburghii.We use Caenorhabditis elegans as a model organism to study the effects of two A.formosanus polysaccharide on lifetime of Caenorhabditis elegans under oxidative stress,then we can evaluate antioxidant activity of A.formosanus and A.zhejiangensis.The results show that:(1)the solvent extraction method is the most effective method to extract polysaccharide of A.roxburghii,polysaccharide content of Anoectochilus zhejiangensisis significantly higher than that of A.formosanus.(2)With the increase of polysaccharide concentration,hydroxyl free radical clearance rate has a tendency to rise,the hydroxyl free radical clearance rate of Anoectochilus zhejiangensispolysaccharide is significantly higher than that of A.formosanus polysaccharide.(3)Both kinds of Anoectochilus polysaccharide can prolong the lifetime of C.elegans,but the impact of two kinds ofpolysaccharide to the lifetime of C.elegans is not obviously different.The results show that both kinds of Anoectochilus are rich in polysaccharide and have antioxidant activity,but the polysaccharide content and antioxidant activity of the two kinds Anoectochilus are different.The results lay agood foundation for the further study of choosing and cultivating the best origin A.roxburghii.更多还原