【摘要】 以紫薇(Lagerstroemia indica)、尾叶紫薇(L.caudata)、屋久岛紫薇(L.fauriei)和福建紫薇(L.limii)4种紫薇属植物为材料,利用染色体荧光原位杂交技术(FISH)获得了4种紫薇属植物的有丝分裂中期染色体FISH图及核型参数,分析了45SrDNA在紫薇属植物染色体上的数量和分布特点。结果表明,4种紫薇属植物染色体上均具有1对45SrDNA杂交位点,位于较长染色体短臂的近端部,紫薇、尾叶紫薇、屋久岛紫薇和福建紫薇的核型公式分别为2n=48=2M+24m+22sm、2n=48=30m+18sm、2n=48=2M+20m+26sm和2n=48=2M+32m+14sm,均为2A型。该研究首次获得了紫薇属植物45SrDNA荧光原位杂交核型,为紫薇属植物亲缘关系研究和细胞生物学研究提供了分子细胞学依据。更多还原

【Abstract】 Fluorescence in situ hybridization(FISH)system was established in Lagerstroemia species,and45 SrDNA loci were anchored on the chromosomes of L.indica,L.caudata,L.fauriei and L.limii.The results showed that only one pair of 45 SrDNA loci were found at the end of the short arms of one pair of homologous chromosomes.The karyotypes of the four Lagerstroemiaspecies were all 2Aand the karyotype formulas were 2n=48=2M+24m+22sm(L.indica),2n=48=30m+18sm(L.caudata),2n=48=2M+20m +26sm(L.fauriei)and 2n=48=2M+32m+14sm(L.limii)respectively.It is the first report on karyotype analysis with 45 SrDNA loci in Lagerstroemia genus and the results will provide a cytomolecular basis for genetic research of Lagerstroemia.更多还原