【摘要】 以对羟基苯硼酸为前驱体,利用过氧化氢（H₂O₂）可以定量氧化对羟基苯硼酸产生对羟基苯酚的原理,以反应产物对羟基苯酚为电化学信号物质,结合金纳米粒子修饰玻碳电极（AuNPs/GCE）,发展了一种间接检测H₂O₂的电化学方法.由于AuNPs/GCE具有有效电子传递性能和比表面积大等优点,对硼酸氧化产物具有较高的催化活性,因此在含1.0 mmol·L^-1对羟基苯硼酸的0.1 mol·L^-1pH 7.5 PBS中,AuNPs/GCE可以检测到1.0¹.0×10³μmol·L^-1的H₂O₂,检测限为0.5μmol·L^-1.同时,该方法具有良好的选择性和重现性,且操作简单、速度快、价格低廉,非常适用于实际样品中H₂O₂含量的测定.更多还原

【Abstract】 We developed a new method for electrochemical detection of hydrogen peroxide（H₂O₂） based on boronate oxidation of p-hydroxyphenylboronic acid. This method using p-aminophenol which is produced from the reaction of H₂O₂ and p-aminophenylboronic acid as a well electrochemical probe, combined with a gold nanoparticles（AuNPs） modified electrode for an indirect detection of H₂O₂. Because of the large surface area and enhanced electrocatalytic behavior by the AuNPs modified electrode, the detection sensitivity was improved. The method could detect H₂O₂ in the concentration range of 1.0 100 μmol·L^-1and 0.1 1.0 mmol·L^-1in 0.1 mol·L^-1pH 7.5 PBS containing 1.0 mmol·L^-1p-hydroxyphenylboronic acid. The low detection limit was0.5 μmol·L^-1. The proposed method had good selectivity and stability. Moreover, the method was quick, simple and cheap, which has potential application in real samples analysis.更多还原