The wild soybean backcross introgressive lines BC_3F_3 was constructed with cultural soybean Suinong 14 as recurrent parent and wild soybean ZYD00006 as donor parent in this study. One hundred and twenty one polymorphism SSR markers between the parents were used to analyze the genotypes of 114 lines. The line with double introgression homozygote loci from wild soybean plants( B-B loci) and Suinong 14 were analyzed with T-test. With P≤0. 05 as threshold value,108 B-B locus were significant on phenotype. And ...