【摘要】 通过低温处理打破细叶百合(Lilium pumilum)鳞茎休眠,利用荧光差异凝胶电泳和质谱技术,借助生物信息学分析手段,分析百合鳞茎休眠解除过程中蛋白质组的变化,以期进一步理解百合鳞茎休眠机制奠定基础。结果表明,分离得到31个差异表达蛋白点;相对于休眠鳞茎,休眠解除鳞茎中上调表达的蛋白点有15个,下调表达的蛋白点有16个;应用MS质谱成功鉴定12个差异蛋白点,按功能划分为6类,主要为胁迫类蛋白,可能涉及鳞茎内物质的代谢过程,进而调控鳞茎的休眠解除;根据细叶百合鳞茎休眠解除过程中差异蛋白表达谱比较,获得了几种与鳞茎休眠相关的蛋白质,鳞茎休眠时胁迫类蛋白高表达,休眠解除时蛋白水解酶类高表达。更多还原

【Abstract】 Lily bulb dormancy was broken during low temperature storage,Fluorescent difference gel electrophoresis,MALDI-TOF/TOF and bioinformatic technology were used in the experiment.A total of 31 differentially expressed proteins were separated by using fluorescent difference gel electrophoresis.Among these proteins,there were 15 proteins up-regulated and 16 proteins down-regulated in the dormancy breaking bulbs.These proteins were classified into 6categories by function.Comprehensive analysis showed that these proteins were mainly stress protein,they may involved in metabolic process of the bulbs,and regulate the bulb dormancy breaking.According to the expression differences of the proteins in dormant and dormancy breaking bulbs,several proteins identified might be related to the process of bulb dormanc.Stress proteins had high expression in dormant bulbs,and proteolytic enzymes had high expression in dormancy breaking bulbs.This research had set the stage for further understanding of the bulb dormancy mechanisms in lily.更多还原