【摘要】 为实现贝西滑刃线虫的高效、准确鉴定,根据贝西滑刃线虫核糖体28SrRNA-D2/D3片段的保守区域设计了特异性引物,并结合植物寄生线虫通用引物作为内标,构建了贝西滑刃线虫的一步双重PCR检测体系。结果表明:特异性引物GU-F/GU-R对贝西滑刃线虫具有高度的特异性,扩增出245bp的特异性目标片段,有效检测灵敏度可达0.125ng/μL线虫DNA模板量。该方法特异性好、灵敏度高、稳定性强,适用于贝西滑刃线虫的快速检测。更多还原

【Abstract】 To detect Aphelenchoides besseyi accurately and effectively,specific primers were designed based on the conserved regions of 28SrRNA-D2/D3 in the ribosome,and combined with the universal primers for the amplification of ribosomal ITS region in plant-parasitic nematodes.The duplex PCR method for detecting A.besseyi was therefore established.The results showed that a fragment of 245 bp was amplified fromA.besseyi by specific primers GUF/GU-R.The sensitivity test showed that the duplex PCR can detect 0.125ng/μL of nematode DNA.The results indicated the duplex PCR method had advantages of high specificity,sensitivity and stability.It can be effectively used in the detection of A.besseyi.更多还原