【摘要】 目的探讨达比加群对凝血酶诱导的人气道平滑肌(HASM)细胞收缩和增殖的影响。方法将原代培养的HASM细胞用于实验。用免疫荧光的方法观察细胞收缩的形态学变化;用Western blot法分析α-actin蛋白相对表达量;用cck8法检测平滑肌细胞增殖能力。结果达比加群能显著抑制凝血酶诱导的HASM细胞骨架重组和收缩蛋白α-actin的表达(P<0.05)。对血管紧张素Ⅱ(AngⅡ)、转化生长因子-β1、血小板源性生长因子、乙酰甲胆碱、凝血酶的促增殖能力进行比较,凝血酶显著强于AngⅡ,仅次于10%胎牛血清(P<0.05)。达比加群呈浓度依赖性和时间依赖性地抑制了凝血酶诱导的HASM细胞增殖(P<0.05)。结论达比加群能够显著抑制凝血酶诱导的HASM细胞收缩和增殖。更多还原

【Abstract】 Objective To investigate the effects of dabigatran on thrombin-induced contraction and proliferation of human airway smooth muscle(HASM)cells.Methods The primary cultured human bronchial smooth muscle cells were used in this experiment.The immunofluorescence method was used to observe the morphological changes of HASM cells contraction,and theα-actin protein relative expression was analyzed by Western blot.The proliferation ability of HASM cells was assessed by cck8 assay.Results Dabigatran could significantly inhibit the thrombin induced HASM cytoskeleton reorganization andα-actin protein expression(P<0.05).In addition,in the comparison of the stimulated proliferation ability of HASM cells among AngⅡ,TGF-β1,PDGF,and Mch and thrombin,thrombin was stronger than AngⅡ,and was second to the 10%FBS(P<0.05).Moreover,thrombin-induced HASM cell proliferation was inhibited by dabigatran in a concentration-dependent and time-dependent manners(P<0.05).Conclusion Dabigatran can significantly inhibit the contraction and proliferation of HASM cells induced by thrombin.更多还原