【摘要】 目的通过模拟人阻塞性睡眠呼吸暂停综合征(OSAS)间歇性低氧(IH)环境,观察其对人外周血来源树突状细胞(DCs)迁徙能力的影响,并通过干预RelB、p38的表达探讨IH致DCs迁徙能力改变的可能通路机制。方法培养前将DCs分为RelB、p38干扰及非干扰质粒组。利用间歇低氧舱设置低氧环境,其中,给氧浓度为0.5%、1.5%、5.0%、10.0%,低氧/再氧合时间比为1∶1、1∶3、1∶5、1∶9,常氧对照组予以持续21.0%氧浓度供应。体外培养结束后蛋白质印迹法检测RelB、p38的表达,侵袭小室检测DCs的迁徙能力。结果相对于常氧,体外IH下DCs整体迁徙能力下降,且DCs迁徙能力与IH环境下平均氧分压水平呈正相关(r=0.867,P<0.05),IH可促进DCs胞内RelB、p38表达,而干预二者表达均未逆转IH下迁徙能力的改变。结论体外IH可导致DCs迁徙能力下降,且可能与RelB、p38激活无关。更多还原

【Abstract】 Objective By simulating the intermittent hypoxia(IH)environment of human obstructive sleep apnea syndrome(OSAS),to reveal the effect of IH on migratory ability of human peripheral blood derived dendritic cells(DCs),and through the intervention of RelB,p38 expression in order to explore the possible mechanism of the change of DCs migration ability induced by IH.Methods DCs were divided into RelB,p38 siRNA interfering and non interfering plasmid group before cultivation.The environment of hypoxia was created by a intermittent hypoxia cabin,among them,oxygen concentration was 0.5%,1.5%,5.0%,10.0%,hypoxia/reoxygenation time ratio was set as 1∶1,1∶3,1∶5and 1∶9,while sustained oxygen was supplied to the contrast at a normal concentration of 21.0%.The content of RelB and p38 was tested by Western blotting after culture in vitro,migration ability of DCs was detected by invasion chamber.Results Compared with normoxia,DCs under IH tended to have declined migratory ability,which was confirmed to be correlated with the average oxygen partial pressure level under IH.IH could promote the expression of RelB and p38 in DCs,while the migratory ability of DCs was not reversed after intervening the expression of RelB and p38.Conclusion IH in vitro could cause a decline in migratory ability of DCs,which may not be induced by activation of RelB or p38 in DCs.更多还原