【摘要】 目的研究在食管正常上皮历经癌前病变发展到食管鳞状细胞癌的过程中,食管间质中的成纤维细胞的α-SMA表型变化。方法免疫组化法分析20例正常食管组织、80例食管癌前病变组织及50例食管癌组织标本中食管间质成纤维细胞α-SMA的表达,细胞培养3种食管间质成纤维细胞,纯化后行细胞免疫染色,RT-PCR法检测三种食管间质成纤维细胞α-SMA的表达。结果免疫组化结果显示,α-SMA在正常食管、食管癌前病变和食管癌组织间质成纤维细胞中的表达具有明显差异;RTPCR法显示,3种食管间质成纤维细胞在mRNAα-SMA的表达量上具有明显差异。结论食管间质成纤维细胞随癌变进展会逐步发生活化,食管癌相关纤维母细胞可能是由正常的食管成纤维细胞发展至不典型增生成纤维细胞,再逐步恶变发展而来。更多还原

【Abstract】 Objective To investigate the expressions ofα-SMA in different esophageal stromal fibroblasts in esophageal carcinogenesis.Methods IHC method was uesd to detect the expression ofα-SMA protein in stromal fibroblasts of twenty normal esophageal tissues,eighty precancerous lesions and fifty esophageal carcinomas respectively.Three kinds of esophageal stromal fibroblasts were cultured primarily and cells immunohistochemical staining was carrired out after being purified.Expression ofα-SMA was detected by RT PCR.Results IHC results showed thatα-SMA expressions in normal,precancerous and cancerous lesions were of significant differences.RT-PCR results showed thatα-SMA expressions were different significantly among three kinds of fibroblasts.Conclusion Esophageal stromal fibroblasts were activated with carcinogenensis.AFs was possibly the origion of CAFs.更多还原