【摘要】 目的探讨机械力刺激对人肺泡上皮A549细胞c-Jun氨基末端激酶/促分裂原活化蛋白激酶(JNK/MAPK)表达的影响。方法建立离体的A549细胞机械刺激模型:分别以大小为1 000μstrain的张应力或压应力刺激细胞,在刺激前和刺激后0、5、15、30、60min时间点进行观察,共10组,每组3皿细胞。采用Western blot检测机械刺激后细胞内磷酸化JNK/MAPK蛋白、总JNK/MAPK蛋白的表达变化。结果 A549细胞经机械力刺激后,磷酸化JNK/MAPK蛋白水平增加。张应力组在30min时间点增加最明显,压应力组在15min时间点最明显。之后随着机械刺激时间延长,张应力和压应力刺激组的磷酸化JNK蛋白水平均出现下降,在60min时下降至基线水平。结论 1 000μstrain张应力或压应力刺激,均激活A549细胞JNK/MAPK,提示JNK通路可能参与了机械力诱导的A549细胞信号转导。更多还原

【Abstract】 Objective To explore whether the JNK/MAPK is involved in the mechanotransduction of the A549 cells induced by mechanical force.Methods Cells were divided into ten groups:tensile stress groups(0,5,15,30,60min),and compressive stress groups(0,5,15,30,60min),3replicate wells in each group.Cells were stimulated by tensile stress or compressive stress respectively at the magnitude of 1 000μstrain for 5,15,30,60 min.Then the protein level of JNK and phosphorylated JNK were tested by Western blot.Results After mechanical stimulation,the protein level of phosphorylated JNK/MAPK increased.These were most obviously at the 30 min time point in both tensile stress group and the 15 min time point in compressive stress group.However,the protein level of phosphorylated JNK/MAPK returned to the original level at the 60 min time point in tensile stress group and the compressive stress group.Conclusion Mechanical force activated the expression of JNK in A549 cells.JNK pathway might be involved in signal transduction in A549 cells induced by mechanical force.更多还原