【摘要】 目的研究CD4~+CD25~+CD127low/-调节性T细胞(Treg)在晚期胃癌患者外周血中的表达水平及其对化疗疗效判断的意义。方法采用流式细胞术(FCM)检测30例晚期胃癌患者和10例健康体检者外周血中CD4~+CD25~+CD127low/-Treg的表达水平。结果晚期胃癌患者外周血中CD4~+CD25~+CD127low/-Treg占CD4~+T淋巴细胞的百分比为(10.41±2.85)%,明显高于健康体检者(5.05±1.32)%(t=8.04,P<0.01),也明显高于化疗2周期后(7.07±1.45)%,(t=5.72,P<0.01)和化疗4周期后(7.30±1.65)%(t=5.17,P<0.01)。晚期胃癌患者化疗2周期后外周血中CD4~+CD25~+CD127low/-Treg占CD4~+T淋巴细胞的百分比为(7.07±1.45)%,与化疗4周期后(7.30±1.65)%比较,差异无统计学意义(t=0.57,P>0.05)。晚期胃癌患者外周血中CD4~+CD25~+CD127low/-Treg表达水平与患者性别、年龄、胃癌相关肿瘤抗原及肿瘤部位无相关性(P>0.05),低分化程度与中分化程度差异无统计学意义(P>0.05)。晚期胃癌患者化疗2周期后缓解组(完全缓解~+部分缓解)外周血CD4~+T细胞中CD4~+CD25~+CD127low/-Treg的水平为(6.25±1.08)%与化疗2周期后未缓解组(病变稳定~+疾病进展)的(7.98±1.27)%比较,差异有统计学意义(t=4.02,P<0.01)。结论晚期胃癌患者外周血中CD4~+CD25~+CD127low/-Treg的表达水平与肿瘤缓解情况有关。更多还原

【Abstract】 Objective To study the expression level and diagnostic value of CD4~+CD25~+CD127low/-regulatory T cells(Treg)in peripheral blood from advanced gastric cancer patients.Methods The expression level of CD4~+CD25~+CD127low/-regulatory T cells in peripheral blood from 30 advanced gastric cancer patients and 10 healthy volunteers was examined by flow cytometry(FCM).Results The population of CD4~+CD25~+CD127low/-regulatory T cells in peripheral blood from advanced gastric cancer patients accounted for(10.41±2.85)% of CD4~+T lymphocytes,which was significantly higher than those from healthy volunteers(5.05±1.32)%(t=8.04,P<0.01),advanced gastric cancer patients after 2cycles of chemotherapy(7.07±1.45)%(t=5.72,P<0.01),and advanced gastric cancer patients after 4cycles of chemotherapy(7.30±1.65)%(t=5.17,P<0.01).The population of CD4~+CD25~+CD127low/-regulatory T cells in peripheral blood from advanced gastric cancer patients after 2cycles of chemotherapy accounted for(7.07±1.45)% of CD4~+T lymphocytes,and there was no significant variations than those from advanced gastric cancer patients after 4cycles of chemotherapy(7.30±1.65)%(t=0.57,P>0.05).There was no relationship between the expression level of CD4~+CD25~+CD127low/-regulatory T cells in peripheral blood from advanced gastric cancer patients and their gender,age,gastric cancer related tumor antigens and the site of the tumor(P>0.05),there was no significant variations between the expression level of CD4~+CD2~+CD127low/-regulatory T cells in peripheral blood from poorly differentiated group and those from moderately differentiated group(P>0.05).The population of CD4~+CD25~+CD127low/-regulatory T cells in peripheral blood from advanced gastric cancer patients who were remission group(CR~+PR)after 2cycles of chemotherapy accounted for(6.25±1.08)% of CD4~+T lymphocytes and those from advanced gastric cancer patients who were no remission group(SD~+PD)after 2cycles of chemotherapy accounted for(7.98±1.27)%,there was difference between the remission group and the no remission group(t=4.02,P<0.01).Conclusion There was relationship between the expression level of CD4~+CD25~+CD127low/-regulatory T cells in peripheral blood from advanced gastric cancer patients after 2cycles of chemotherapy and the tumor response.更多还原