【摘要】 通过体外细胞模型,考察蚯蚓小分子多肽提取物(LMWPEE)对正常成人皮肤成纤维细胞(HSF)和正常成人表皮角质形成细胞(HEK)的细胞活性以及对HSF分泌Ⅰ型前胶原蛋白的影响。结果表明,与空白组相比,质量浓度为25~400 mg/L的LMWPEE对2种细胞的细胞活性都具有显著促进作用,但对2种细胞的细胞周期并没有明显的影响。LMWPEE对HSF细胞活性的促进作用存在一定的剂量-效应关系,而促进HEK细胞活性的较佳质量浓度为50 mg/L。此外,LMWPEE在质量浓度为200和400 mg/L时,可显著促进HSF分泌Ⅰ型前胶原蛋白,较空白组分别提高了31%(P<0.05)和51%(P<0.01)。更多还原

【Abstract】 The effect of low molecular weight peptides extract from Earthworm( LMWPEE) on cell viability in cultured normal adult human skin fibroblasts( HSF) and normal adult human epidermal keratinocytes( HEK)was studied,separately.Its effect on synthesis of type I procollagen in cultured HSF was also studied.The results showed that,as comparing with the control group,the LMWPEE with mass concentrations between 25 and 400 mg /L significantly increases cell viability of HSF and HEK,but it has no effect on cell cycle of HSF and HEK.The effect of LMWPEE on HSF cell viability shows a certain dose-effect relationship,while best dosage of LMWPEE for HEK cell viability is 50 mg /L.LMWPEE remarkably increases the production of type I procollagen in cultured HSF.The production of type I procollagen increases by 31%( P < 0.05) and 51%( P < 0.01) at mass concentration 200 and 400 mg /L of LMWPEE as compared with control group,respectively.更多还原