【摘要】 目的探讨血管内皮生长因子、血管生成素-2和survivin在非小细胞肺癌患者血清中的表达及相关性研究。方法采用夹心ELISA法分别检测112例非小细胞肺癌患者和28例健康志愿者血清中血管内皮生长因子、血管生成素-2和survivin的表达情况。结果与对照组比较,肺癌组血管内皮生长因子、血管生成素-2和survivin水平均增高,差异有统计学意义(P<0.01),血清中血管内皮生长因子、血管生成素-2和survivin的表达与患者的年龄、性别无明显相关(P>0.05),而与淋巴结转移和分期显著相关(P<0.05),腺癌患者血清中survivin的水平明显高于鳞癌患者(P<0.05)。血清中血管内皮生长因子的表达与血管生成素-2和survivin显著相关(P<0.05)。结论血清中血管内皮生长因子、血管生成素-2和survivin可作为肺癌转移和判断分期的良好指标,联合检测可能有助于非小细胞肺癌的早期诊断。更多还原

【Abstract】 Objective To investigate the expression of VEGF、Ang-2and survivin in lung carcinoma and the relationship between VEGF expression and Ang-2、survivin expression.Methods A total of 112 cases of malignancies in lung and 28 cases of healthy volunteers were detected for sera VEGF、Ang-2and survivin with monoclonal antibody against human.Results The levels of VEGF、Ang-2and survivin in lung carcinoma group were higher than those in control group(P<0.01).Sera VEGF、Ang-2and survivin expression didn’t have significant relationship with patient sex and age(P>0.05),but had significant relationship with lymph node metastasis and stage(P<0.05).And the level of survivin in lung adenocarcinoma was higher than in squamous cell carcinoma(P<0.05).The Sera VEGF expression has significant relationship with the Ang-2and survivin expression(P<0.05).Conclusion Sera VEGF、Ang-2and survivin may be good predicator for metastasis and judge of stage of lung carcinoma,and it may contribute to the diagnosis of lung carcinoma with combined determination.更多还原