【摘要】 目的:筛选调控黄芪次生代谢产物皂苷含量诱导子。方法:利用正交试验设计,以黄芪皂苷含量为衡量指标,对调控相应代谢途径的诱导子进行了筛选。结果:水杨酸(SA)、茉莉酸甲酯溶液(Me JA)进行地上部位处理3d的处理组合是对黄芪叶皂苷含量影响的最佳处理组合;硝酸银(Ag NO3)、Me JA进行地上部位处理3d的处理组合是对黄芪茎皂苷含量影响的最佳处理组合;水及内生菌发酵液溶液进行地下部位处理3d的处理组合是对黄芪根皂苷含量影响的最佳处理组合。结论:黄芪皂苷代谢途径的最佳诱导子是Me JA,处理部位是地下部位,最佳处理时间是3d,最佳皂苷含量变化检测部位是黄芪根部。更多还原

【Abstract】 Objective: To screen the regulation elicitor of secondary metabolite of astragalus saponins. Methods: Using the orthogonal experiment design, saponins content of astragalus were used to measure the for screening the regulation elicitor of metabolic pathways. Results: The treatment of 3 days of treatment with the combination of salicylic acid（SA） and methyl Jasmonale（Me JA） was the best combination of the effects of the concentration of astragalus leaves, the treatment of the 3 days of treatment with the combination of the treatment of silver nitrate（AgNO₃） and Me JA was the best combination of the effects of the content of the stem saponins of astragalus, in the treatment of 3 days, the combination of the treatment of days in the fermentation broth of endophytic bacteria was the best treatment combination of the effects of the content of the saponins. Conclusion: Me JA is the best elicitor of flavones secondary metabolic pathways, the best processing part is underground part, the optimum processing time is 3 days, and best detection part is root.更多还原