【摘要】 目的:本研究通过糖尿病大鼠结肠生物力学重构与糖基化终末产物表达的相关性来探讨肠润通的干预作用机制。方法:将成年健康SD大鼠40只,每组10只随机分为空白对照组,糖尿病对照组,肠润通高(50g/kg)、低(25g/kg)剂量治疗组。肠润通每日1次经灌胃给药,定期检测血糖和体重,实验周期为60d。实验结束时,通过免疫组织化学染色方法观测各组糖基化终末产物(AGEs)及其受体(RAGE)在结肠肠壁各层的分布表达,利用图像分析软件对其表达进行定量分析。结肠管壁的湿重,壁厚、展开角、残余应变以及应力应变等形态学和生物力学参数取自本课题组之前相关研究报道的结果。采用线性回归方法对上述两组参数进行相关统计分析。结果:糖尿病对照组AGEs和RAGE在结肠肠壁各层的表达明显高于空白对照组(P<0.05,P<0.01)且与形态组织学和生物力学重构参数高度正相关。经肠润通治疗后,高剂量肠润通处理能显著降低AGEs和RAGE在结肠肠壁各层的表达(P<0.05,P<0.01),但低剂量肠润通处理无显著作用。结论:AGEs和RAGE在肠壁各层组织的表达与糖尿病结肠生物力学特性重构高度相关,高剂量肠润通对AGEs-RAGE表达的抑制作用是糖尿病结肠生物力学特性重构机制改善的有效途径之一。更多还原

【Abstract】 Objective:The former research demonstrated that the high dosage of Changruntong(CRT) could partly restore the morphological and biomechanical reconstruction of colon in diabetic rats.In this study,the correlation between biomechanical reconstruction and expression of advanced glycation end products(AGEs) of colon in the diabetic rats was analyzed,in order to investigate the intervention mechanism of CRT.Methods:Forty SD rats were randomly divided into normal group,diabetes mellitus group,high(50g/kg) and low(25g/kg) dosage of CRT groups,and with 10 rats in each group.Rats in high and low dosage of CRT groups received gavage administration with CRT daily,and the modeling process lasted for 60 days.Blood glucose level and body weight of rats were measured termly.The expression of AGEs and RAGE in colon bowel layers was detected by using immunohistochemistry and analyzed quantificationally by using imaging analysis software.The morphological and biomechanical parameterssuch as wet weight,thickness,opening angle,residual strain and stress-strain of colonic wall were referenced to the former research reports.The correlation between the above two groups of parameters was analyzed by using linear regression analysis.Results:The expression of AGEs and RAGE in colon bowel layers of rats in DM group was higher than that of normal group(P<0.05,P<0.01),and the AGE/RAGE expressions were highly and positively correlated with morphological and biomechanical parameters.The expression of AGE and RAGE was significantly decreased in colon bowel layers of ratsin high dosage of CRT group(P<0.05,P<0.01),but the expression of AGE and RAGE in colon bowel layers of ratsin low dosage of CRT group was not changes significantly.Conclusion:The expression of AGE and RAGE in colon bowel layers is highly correlated with the biomechanical reconstruction of colonin diabetic rats,and the inhibitory effect of high dosage of CRT on AGE/RAGE expression might be one of the effective approaches for improving the morphological and biomechanical reconstruction of colon in diabetic rats.更多还原