【摘要】 目的:研究金欣口服液对RSV感染大鼠体内Ⅰ型干扰素相关蛋白STAT1、STAT2和P-STAT1、P-STAT2表达的调控趋势。方法:将大鼠分为正常对照组(A组)、感染模型组(B组)、金欣口服液低剂量组(C组)、金欣口服液中剂量组(D组)、金欣口服液高剂量组(E组)、预防组(F组)、利巴韦林组(G组)7组,成功造模后,分别采用ELISA和RT-PCR检测各组大鼠肺组织内P-STAT1、P-STAT2和STAT1、STAT2的表达。结果:感染模型组较正常对照组、预防组和金欣口服液低剂量组未能上调大鼠体内P-STAT1和P-STAT2蛋白表达;而金欣口服液中、高剂量及利巴韦林组明显上调感染模型组RSV大鼠体内的P-STAT1和P-STAT2蛋白的表达(P<0.05,P<0.01)。结论:实验表明金欣口服液可通过上调P-STAT1和P-STAT2的表达,诱导IFN的产生,发挥抗病毒作用。更多还原

【Abstract】 Objective:To study the regulatory effect of Jinxin Oral Liquid on type I interferon related proteins such as STAT1,STAT2,P-STAT1 and P-STAT2 in RSV infected rats.Methods:rats were randomly divided into control group,model group,low,middle and high dosages of Jinxing Oral Liquid groups,prevention group and ribavirin group.After establishing the rat models,Elisa and RT-PCR were used to test the expression of P-STAT1,P-STAT2,STAT1 and STAT2 in lung tissue of rats in all the groups.Results:The expression of P-STAT1 and P-STAT2 of rats in low dosage of Jinxing Oral Liquid groups and prevention group was not increased,and the expression of P-STAT1 and P-STAT2 of rats in middle and high dosages of Jinxing Oral Liquid groups and ribavirin group was increased significantly(P<0.01).Conclusion:The results show that Jinxing Oral Liquid could induce the occurrence of IFN and play the role of antiviral by up-requlatinq the expression of P-STAT1 and P-STAT2.更多还原