【摘要】 目的:探索一种对人体无害、无痛、操作简便的获取基因组脱氧核糖核酸(deoxyribonucleic acid,DNA)的方法。方法:从唾液中提取基因组DNA,应用聚合酶链式反应(polymerase chain reaction,PCR)扩增血红蛋白β基因,采用含溴乙锭的琼脂糖凝胶电泳鉴定扩增产物。结果:从唾液中提取基因组DNA能够扩增出血红蛋白β基因。结论:唾液作为获取基因组脱氧核糖核酸的简易来源,取样方法简便、快捷,更适用于疾病研究中大规模群体筛查。更多还原

【Abstract】 Objective: To explore a harmless,painless and easily- operated method for genomic deoxyribonucleic acid( DNA). Methods: Genomic DNA was extracted from saliva. Polymerase chain reaction( PCR) was applied to amplify hemoglobin β gene,and the amplification products were identified by agarose gel electrophoresis containing ethidium bromide. Results: The genomic DNA extracted from saliva can amplify the hemoglobin β gene. Conclusion: Saliva is a simple source of genomic DNA. The method is simple and fast,and may be used for screening on a large scale.更多还原