【摘要】 目的:检测人非小细胞肺癌(NSCLC)组织标本中神经纤毛蛋白1(NRP1)mRNA和miR-9的表达水平,并探讨二者表达与NSCLC患者临床特征之间的关系。方法:在患者知情同意下,收集2010—2011年吉林大学中日联谊医院匹配的NSCLC患者组织标本,包括45例正常组织、45例癌旁组织及45例肿瘤组织。采用qRT-PCR法检测不同组织中NRP1mRNA和miR-9表达水平,分析二者表达水平与不同临床病理特征的相关性。结果:与正常组织比较,癌旁组织中NRP1mRNA表达水平无明显变化(P>0.05),而肿瘤组织中NRP1mRNA表达水平明显降低(P<0.05);与正常组织比较,癌旁组织miR-9表达水平无明显变化(P>0.05),而肿瘤组织中miR-9表达水平明显升高(P<0.05)。男性癌旁组织中miR-9表达水平低于女性(P<0.05)。NSCLC患者肿瘤组织中NRP1mRNA表达水平与性别、年龄、分化程度、TNM分期及临床分期无关联(P>0.05),但与病理分型及淋巴结转移有关联(P<0.05);NSCLC患者肿瘤组织中miR-9表达水平与年龄、肿瘤病理类型、淋巴转移、分化程度、TNM分期及临床分期无关联(P>0.05),而与性别因素有关联(P<0.05)。结论:NSCLC患者肿瘤组织中miR-9表达水平显著升高,而NRP1mRNA表达水平明显下降,NRP1mRNA检测有助于判断NSCLC的分型及转移。更多还原

【Abstract】 Objective:To detect the expression levels of neuropilin1(NRP1)mRNA and miR-9in non-small cell lung cancer(NSCLC)tissue samples,and to explore the correlations between the expressions of NRP1 mRNA,miR-9and the clinicopathological characteristics of the patients with NSCLC.Methods:Informed consent was obtained from each patient before surgery.The tissue samples including 45 NSCLC tissue,45 adjacent carcinoma tissue and 45 normal lung tissue were collected from China-Japan Union Hospital of Jilin University from 2010to2011.qRT-PCR was used to detect the expression levels of NRP1 mRNA and miR-9in three kinds of lung tissue,and the correlation between the expressions of NRP1 mRNA,miR-9and clinicopathological characteristics of the patients with NSCLC was analyzed.Results:Compared with normal tissue,the expression level of NRP1 mRNA in adjacent carcinoma tissue had no change(P>0.05),but the expression level of NRP1 mRNA in non-small cell lung cancer tissue was significantly decreased(P<0.05).Compared with normal tissue,the expression level of miR-9in adjacent carcinoma tissue had no change(P>0.05),but the expression level of miR-9in non-small cell lung cancer tissue was significantly increased(P <0.05).Furthermore,in adjacent carcinoma tissue,the expression level of miR-9in the males was lower than that in the females(P<0.05).In NSCLC tissue,the expression level of NRP1 mRNA had no relationship with sex,age,differentiation degree,TNM stage and clinical stage,but was significantly correlated to the histological subtype and lymph node metastasis(P<0.05).In NSCLC tissue,the expression level of miR-9had no relationship with age,pathological type,lymph node metastasis,differentiation degree,TNM stage,and clinical stage(P>0.05),but was correlated to the sex(P<0.05).Conclusion:The expression level of miR-9is up-regulated and the expression level of NRP1 mRNA is downregulated significantly in non-small cell lung cancer tissue.The detection of the expression level of NRP1 mRNA contributes to judge the histological subtype and lymph node metastasis of NSCLC.更多还原