【Objective】The aims of the paper are to investigate the correlation between in vitro maturation(IVM)and lipid droplets of oocytes from different forms of porcine ovarian and the effect of berberine(Ber).【Methods】Porcine oocytes of GV phase from follicular ovarian and luteal ovarian were matured in IVM medium with Berberine,to observe the effect of IVM;The lipid droplet content of oocytes were measured by the method of Nile red before and after IVM and the distribution of lipid droplet of oocytes of each gro...