【Objective】To investigate the extraction methods of clove oil from clove buds and its anti bacterial effects.【Method】Clove oil was extracted with bath-shocking or ultrasonic extractions.The best extraction conditions were accessed with orthogonal experimental design.Eugenol was identified as effective chemical of clove oil by ultraviolet spectrophotometer and high performance liquid chromatography(HPLC).The amount of eugenol determined by HPLC was 48μg/mL.The antibacterial properties of the extracted clove ...