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欧李优系试管苗增殖关键技术研究
Key Proliferation Technology of Prunus humilis Fine Strains Plantlets in vitro
【摘要】 以欧李优系试管苗为试材,采用单因素试验设计,研究了不同生长调节物质组合、基本培养基类型、附加物和蔗糖浓度对试管苗生长状态和增殖效率的影响。结果表明:在MS+6-BA 0.5mg·L-1+KT 0.5mg·L-1+IBA 0.05mg·L-1培养基上,附加水解乳蛋白(LH)300mg·L-1、蔗糖35g·L-1、琼脂5.8g·L-1,为欧李优系试管苗增殖的最佳培养条件。
【Abstract】 By single factor text,the effects of various basic culture medium,growth regulator combination,additions and sucrose concentration were studied on growth status and proliferation rate of Prunus humilis fine strains invitroplants.The results indicated that the optimal medium of proliferation was MS+6-BA 0.5mg·L-1+KT 0.5mg·L-1+IBA0.05mg·L-1,additional LH 300mg·L-1,sugar 35g·L-1,agar 5.8g·L-1.
【关键词】 欧李;
试管苗;
增殖;
关键技术;
【Key words】 Prunus humilis; plantlets in vitro; proliferation; key technology;
【Key words】 Prunus humilis; plantlets in vitro; proliferation; key technology;
【基金】 邯郸市科技局资助项目(1327101071-2)
- 【文献出处】 北方园艺 ,Northern Horticulture , 编辑部邮箱 ,2016年17期
- 【分类号】S662.5
- 【被引频次】2
- 【下载频次】113