【摘要】 以野生青海双孢菇（Agaricus bisporus（Lange）Imbach）为试材,以胞外多糖含量为响应值,在单因素试验的基础上,采用4因素4水平响应面分析法,研究了培养基中不同营养因子对其菌丝代谢胞外多糖含量的影响,以确定最佳野生双孢菇发酵培养基。结果表明:最优培养基为甘露醇19.83g·L^-1,蛋白胨6.35g·L^-1,维生素B121.61g·L^-1,硫酸亚铁0.42g·L^-1,胞外多糖含量最高可达2.002g·L^-1,与试验模型预测值基本一致。更多还原

【Abstract】 Taking Agaricus bisporus（Lange）Imbach as material,the content of extracellular polysaccharide as the response value,on the basis of single factor experiment,the effect of different nutritional factors on the content of extracellular polysaccharide were researched by the response surface methodology with 4 factors and 4 levels to determined the optimum culture medium.The results showed that the best culture medium for fermentation extracellular polysaccharide of the Agaricus bisporus（Lange）Imbach was mannitol 19.83g·L^-1,peptone 6.35g·L^-1,vitamin B121.61g·L^-1,iron sulfate 0.42g·L^-1,the highest production of extracellular polysaccharide reached 2.002g·L^-1,which was identical to the response surface prediction value.更多还原