【摘要】 目的:探讨晚期恶性肿瘤患者化疗前后外周血T淋巴细胞亚群(CD3~+、CD3~+CD4~+、CD3~+CD8~+)数值的变化及临床意义。方法:收集50例晚期恶性肿瘤患者,根据病种及临床分期进行标准方案化疗;采集患者首次化疗前和2个周期化疗后外周血标本,通过流式细胞仪检测T淋巴结细胞亚群在化疗前后的数值及比值,分析T淋巴细胞亚群水平变化。结果:50例晚期恶性肿瘤患者2个周期化疗后外周血CD3~+、CD3~+CD4~+T淋巴细胞数目均显著低于首次化疗前(P<0.01)。CD3~+CD8~+T淋巴细胞数目在2个周期化疗后均高于首次化疗前(P<0.01);CD3~+CD4~+/CD3~+CD8~+细胞比值在2个周期后均明显降低(P<0.01)。结论:晚期恶性肿瘤患者可以通过监测外周血中T淋巴细胞亚群数目评估患者免疫功能状态,并指导治疗及判断预后。更多还原

【Abstract】 Objective: To explore the number change of the peripheral blood T lymphocyte subsets( including CD3~+,CD3~+CD4~+and CD3~+CD8~+) and its clinical significance in advanced malignant tumor patients before and after chemotherapy. Methods: The standard chemotherapy in 50 advanced malignant tumor patients were implemented according to the disease kind and clinical stage. The peripheral blood was collected before chemotherapy and after 2 cycles of chemotherapy. The number and ratio of T lymph node cell subsets were detected using flow cytometry,the relationship between the level change of T lymph node cell subsets was analyzed.Results: The number of CD3~+and CD3~+CD4~+T lymphocyte after 2 cycles of chemotherapy were significantly lower than that before chemotherapy in advanced malignant tumor patients( P < 0. 01). The number of CD3~+CD8~+T lymphocyte after 2 cycles of chemotherapy were higher than that before chemotherapy( P < 0. 01). The ratio of CD3~+CD4~+to CD3~+CD8~+cells after 2 cycles of chemotherapy decreased significantly( P < 0. 01). Conclusions: Monitoring the number of peripheral blood T lymphocyte subsets in advanced malignant tumor patients can assess the immune function,guide treatment and predict prognosis.更多还原