【摘要】 为了高效处理工业含酚废水,研究了波茨坦短芽孢杆菌降解双底物体系过程中间甲酚和4-氯酚的相互作用及苯酚对间甲酚和4-氯酚降解的影响。结果表明,在间甲酚-4-氯酚体系中,4-氯酚会抑制间甲酚的降解,4-氯酚初始质量浓度为40 mg/L时,160 mg/L的间甲酚降解时间延长了8 h;同时间甲酚也抑制4-氯酚的降解,间甲酚初始质量浓度为40 mg/L时,160 mg/L的4-氯酚降解时间延长了4 h。采用Abuhamed动力学方程可以准确描述间甲酚-4-氯酚双底物降解体系中细胞生长的过程,动力学参数I_1,2=1.77,I_2,1=2.47,决定系数R²=0.96。拟合参数表明,4-氯酚对间甲酚降解的抑制要强于间甲酚对4-氯酚降解的抑制。酶活测定表明,底物抑制作用增强时苯酚羟化酶和邻苯二酚1,2-双加氧酶的活性降低。添加低质量浓度的苯酚会对间甲酚和4-氯酚的降解产生促进作用,最佳促进质量浓度为200mg/L;添加300 mg/L以上的苯酚会对间甲酚和4-氯酚的降解产生抑制作用,抑制作用随苯酚质量浓度升高而增强。更多还原

【Abstract】 This paper is aimed to investigate and explore the biodegradation characteristics of m-cresol and 4-chlorophenol in the double-component mixture by using the Brevibacillus borstelensis and their kinetics in hoping to define the effect of phenol on the biodegradation of m-cresol and 4-chlorophenol. To achieve the purpose,we have conducted a batch of experiments and tests in the form of flask cultivation and analysis of phenol,m-cresol and4-chlorophenol via HPLC. The experimental results show that the biodegradation capability of m-cresol tends to be inhibited at presence of 4-chlorophenol. The degradation of 160 mg/L m-cresol can be delayed for 8 h,20 h and 36 h when 40 mg/L,80 mg/L and 120 mg/L 4-chlorophenol were to be added,respectively.Meanwhile,the degradation of 4-chlorophenol would be totally inhibited at presence of m-cresol. Specifically,the delaying time of160 mg/L 4-chlorophenol can be 4 h,16 h and 28 h in the presence of 40 mg/L,80 mg/L and 120 mg/L m-cresol,correspondingly. In addition,we have also adopted a kinetical equation by Abuhamed,which can help to simulate cell growth adequately in the dual-substrate biodegradation system and the interaction parameters can be gained as the result of I_1,2= 1. 77 and I_2,1= 2. 47 with R²= 0. 96. The inhibition rate of 4-chlorophenol on m-cresol proves to be stronger than that of m-cresol on the 4-chlorophenol according to the fitting coefficients. What is more,the specific enzyme activities of the phenol hydroxylase and catechol 1,2-dioxygenase in the mixture of 160 mg/L m-cresol and 120 mg/L4-chlorophenol have been found equal to（ 0. 115 ± 0. 010） U/mg and（ 0. 102 ± 0. 006） U/mg protein,respectively,both of which are lower than those in the single substrate solution of 160 mg/L m-cresol and 120 mg/L 4-chlorophenol. The enzyme assay reveals that,by adding another toxic compound to it,it would be possible to increase the inhibitive power on the enzyme activity,which may lead to lower metabolism of the phenolic compounds.Further exploration helps us to notice that adding 100 mg/L and200 mg/L of phenol may help to speed up obviously the biodegradation of m-cresol and 4-chlorophenol. For instance,in the presence of 200 mg/L phenol,the total degradation time has been found to be 64 h for 160 mg/L m-cresol and 68 h for 120 mg/L 4-chlorophenol,which implies an 8 h and 12 h shorter than that for the dual-substrate mixture,accordingly. But,if increasing the phenol concentration further to 300 mg/L and above,the biodegradation efficiency of m-cresol and 4-chlorophenol may tend to be inhibited and therefore lowered. Moreover,the inhibitive function tends to be increased with the addition of phenolic compounds.更多还原