采用改良的高效液相色谱方法,以贡菊为对照,测定了不同品系昆仑雪菊中绿原酸、木犀草苷、3,5-O-二咖啡酰基奎宁酸及槲皮苷的含量。结果表明,较适宜的高效液相色谱条件为Alitima HP C18 5μm色谱柱(250mm×4.6 mm),以乙腈-0.1%磷酸水溶液梯度洗脱,流速1 m L·min-1,检测波长为350 nm,柱温40℃,进样量5μL。不同品系的昆仑雪菊与贡菊主要活性成分相似,但其含量差异显著。用该方法测得昆仑雪菊和贡菊中的绿原酸、木犀草苷、3,5-O-二咖啡酰基奎宁酸及槲皮苷的含量均高于药典规定的含量标准,其中昆仑雪菊中绿原酸、木犀草苷和槲皮苷的含量显著高于供试贡菊。
【英文摘要】
The contents of chlorogenic acid, galuteolin, 3, 5-O-caffeoylquinic acid, and quercitrin in different strains of Kunlun Chrysanthemum(Coreopsis tinctoria) were determined using a modified high performance liquid chromatography(HPLC) method with Florists Chrysanthemum as the control. The results showed that the suitable HPLC conditions were: Alitima HP C18 5 μm chromatographic column(250 mm×4.6 mm), acetonitril-0.1%phosphoric acid aqueous gradient elution, flow rate of 1 m L·min-1, detection wavelength of 35...