[Objective] The aim was to study pollen cryopreservation approaches of Iris ensata,which had a great significance in solving the problem of asynchronous flowering period on cross breeding and indigenous resources conservation. [Method]Using Iris ensata which is a coldresistant species and has high ornamental value as material,the approaches of its pollens cryopreserving,the viability detection of hanging drop culture and the screening of vitro culture medium were studied. [Result]The results showed that the...