【摘要】 目的观察滋阴泻火中药对达那唑诱导的雌性性早熟模型大鼠青春期启动时下丘脑生长激素释放肽(Ghrelin)及其受体(growth hormone secretion peptide receptor 1α,GHSR1-α)表达的影响。方法将40只雌性SD大鼠随机分为正常组、模型组、生理盐水干预组及中药干预组,每组10只。模型组、生理盐水干预组及中药干预组于大鼠5日龄时一次性皮下注射达那唑300μg制备性早熟大鼠模型。15日龄起中药干预组、生理盐水干预组分别予滋阴泻火中药及生理盐水灌胃,给药干预7~10天,正常组不予处理。记录阴门开启情况,计算卵巢及子宫指数,检测外周血雌二醇(E2)、卵泡雌激素(FSH)、黄体生成素(LH)、下丘脑促性腺激素释放激素(GnRH)含量、下丘脑Ghrelin及其受体GHSR1-α基因表达水平。结果与正常组比较,模型组大鼠阴门开启时间提前,外周血E2、LH水平、子宫指数、下丘脑GnRH含量升高,外周血FSH、下丘脑Ghrelin及GHSR1-αmRNA水平降低(P<0.05,P<0.01);与模型组及生理盐水干预组比较,中药干预组大鼠阴门开启时间未提前,外周血E2、LH水平、子宫指数及下丘脑GnRH含量明显降低(P<0.05,P<0.01),而下丘脑Ghrelin及GHSR1-αmRNA水平升高(均P<0.01);4组间下丘脑Ghrelin及受体个数及活性比较,差异无统计学意义(P>0.05)。结论在基因转录水平上滋阴泻火中药对性早熟大鼠下丘脑Ghrelin及其受体有一定的调节作用。更多还原

【Abstract】 Objective To observe the effect of nourishing yin removing fire Chinese herbs( NYRFCH) on the gene expression of hypothalamic growth hormone secretion peptide( Ghrelin) and its receptor growth hormone secretion peptide receptor 1α( GHSR1-α) at the puberty onset of danazol induced female precocious rats. Methods Forty female SD rats were randomly divided into 4 groups,i. e.,the normal group( N),the model group( M),the normal saline intervention group( NS),and the NYRFCH intervention group( NI),10 in each group. 300 μg danazol was subcutaneously injected to all rats except those in the N group to prepare precocious rat model. NYRFCH and normal saline was respectively administered to rats in the NI and the NS group from the 15 th day old for 7- 10 days. No treatment was given to rats in the N group. Time of rats’ vulva opening was recorded. Ovary index and uterus index were calculated. Peripheral blood levels of estradiol( E2),follicle stimulating hormone( FSH),and luteinizing hormone( LH),and hypothalamic contents of gonadotropin releasing hormone( GnRH) as well as the gene expression of hypothalamic Ghrelin and GHSR1-α were determined. Results Compared with the N group,the vulva opening time was advanced in the model group; peripheral blood levels of E2 and LH,uterus index,hypo-thalamic contents of GnRH increased; peripheral blood FSH levels and mRNA levels of hypothalamic Ghrelin and GHSR1-α decreased(P < 0. 05,P < 0. 01). Compared with the M group and the NS group,the vulva opening time was not advanced in the NI group; peripheral blood levels of E2 and LH,uterus index and hypothalamic contents of GnRH obviously decreased(P <0. 05,P < 0. 01); mRNA levels of hypothalamic Ghrelin and GHSR1-α increased( all P < 0. 01). But there was no statistical difference in the hypothalamic contents of Ghrelin,or the number and activity of GHSR1-α(P > 0. 05). Conclusion NYRFCH had regulatory effect on regulating hypothalamic Ghrelin and GHSR1-α at gene transcription levels.更多还原