【摘要】 目的观察人参总皂苷(total ginsenosides,TG)对野百合碱(monocrotaline,MCT)所致大鼠右心室肥厚的影响并探讨其与钙调神经磷酸酶(Ca N)信号通路的关系。方法将50只雄性SD大鼠随机分为正常对照组,MCT模型组,TG低、中、高剂量组,每组10只,各给药组动物均腹腔注射给药18天。用药前后测定各组大鼠的右心室收缩压(RVSP)、右室肥厚指数(RVHI)、右心重/体重(RVW/BW);Ca2+荧光指示剂Fura-2/AM测定心肌细胞内Ca2+浓度;Real time-PCR检测心肌组织心房利钠因子(ANF)、Ca N mRNA的表达,Western blot检测心肌组织Ca N蛋白表达。结果与MCT模型组比较,TG低、中、高剂量预防给药均使RVSP、RVHI、RV/BW及ANFmRNA表达明显降低,显著降低心肌细胞Ca2+浓度,以及心肌组织Ca N mRNA和Ca N蛋白表达。结论 TG能明显改善MCT诱导的大鼠右心室肥厚,其抗心肌肥厚作用至少与抑制Ca N信号转导通路有关。更多还原

【Abstract】 Objective To observe the effect of total ginsenosides( TG) on monocrotaline( MCT) induced right ventricular hypertrophy rats,and to explore its correlation with calcineurin( Ca N) pathway. Methods Fifty male Sprague Dawley rats were randomly divided into the normal control group,the MCT model group,and the low,middle,high dose TG treatment groups,10 in each group. All medication was performed by peritoneal injection for 18 days. Right ventricular peak systolic pressure( RVSP),right ventricular hypertrophy index( RVHI),and right ventricular weight / body weight( RVW / BW) were measured. Intracellular free calcium concentrations were measured by Ca2 +fluorescence indicator Fura2 / AM. The atrial natriuretic factor( ANF) and Ca N mRNA expression of the myocardial tissue were quantitatively analyzed by Real-time PCR. The protein expression of Ca N was detected by Western blot. Results Compared with the MCT model group,preventive treatment of TG at the 3 doses could significantly reduce RVSP,RVHI,RVW / BW,and ANF mRNA expression,and decrease Ca2 +concentration in myocardial cells,Ca N mRNA and protein expression in the myocardial tissue. Conclusion TG could obviously improve MCT-induced right ventricular hypertrophy,which was possibly achieved through suppressing MCT-activated Ca N signal transduction.更多还原