【摘要】 目的 :探讨A20基因[又称肿瘤坏死因子α诱导蛋白3(tumor necrosis factor alpha-induced protein 3,TNFAIP 3)基因]在肝细胞癌组织中的表达及其与预后的关系。方法 :选取86例原发性肝癌患者的手术切除标本(包括癌及癌旁组织)及26例肝血管瘤患者的正常肝组织标本,采用实时荧光定量PCR法检测A20在肝癌组织及其配对的癌旁组织和正常肝组织中的m RNA表达水平,并分析A20 m RNA表达水平与肝癌患者临床病理特征及接受根治性切除术后预后的关系。结果 :肝癌组织中A20 m RNA的相对表达水平显著高于血管瘤正常肝组织(1.620±0.155 vs 0.410±0.043,P<0.001);对应癌旁组织中A20 m RNA的表达水平(1.215±0.134)也显著高于血管瘤正常肝组织(P=0.001),但与肝癌组织相比的差异无统计学意义(P=0.087)。肝癌组织中A20的表达水平与患者肿瘤分化程度明显相关(P<0.001)。肝癌组织中A20高表达的患者术后总生存(overall survival,OS)率显著低于A20低表达组(P=0.016),至复发时间(time to recurrence,TTR)显著短于A20低表达组(P=0.011)。单因素分析表明,A20表达水平与肝癌患者的预后密切相关(OS:P=0.012;TTR:P=0.013);多因素分析表明,A20表达是影响肝癌患者生存与复发的独立预后因素(OS:P=0.021;TTR:P=0.018)。结论 :肝癌组织中A20 m RNA的表达水平显著高于正常肝组织。A20表达可能成为肝癌患者生存与复发的独立预后因素。更多还原

【Abstract】 Objective: To investigate the expression of A20, also known as tumor necrosis factor alpha-induced protein 3(TNFAIP3) in hepatocellular carcinoma(HCC), and to explore its relationship with prognosis.Methods: The expression levels of A20 m RNA in 86 cases of HCC and the corresponding adjacent tissues as well as 26 cases of normal liver tissues from the patients with hepatic hemangioma were measured by real-time fluorescent quantitative-PCR. The correlations of A20 expression with clinicopathologic features and prognosis of HCC patients after radical operation were analyzed.Results: The relative expression level of A20 m RNA in HCC tissues was 1.620±0.155, which was significantly higher than that in normal liver tissues(0.410±0.043, P < 0.001). The relative expression level in A20 m RNA in para-HCC tissues was 1.215±0.134, which was significantly higher than that in normal liver tissues(P = 0.001). There was no significant difference in A20 m RNA expression level between HCC and para-HCC tissues(P = 0.087). The expression level of A20 in HCC tissues was closely correlated with tumor differentiation(P < 0.001). Kaplan-Meier analysis showed that HCC patients with high expression of A20 m RNA had poorer prognosis with lower probability of overall survival(OS, P = 0.016) and shorter time to tumor recurrence(TTR, P = 0.011). The univariate analysis showed that the expression level of A20 m RNA was closely related to the prognosis of HCC patients(OS: P = 0.012; TTR: P = 0.013). The multivariate analysis showed that A20 could be an independent prognostic factor for HCC patients(OS: P = 0.021; TTR: P = 0.018).Conclusion: The expression level of A20 m RNA in HCC is significantly higher than that in normal liver. A20 may be used as an independent prognostic indicator for survival and recurrence of HCC patients.更多还原