【摘要】 目的:采用核磁共振技术、TTC染色方法检测葛根素预处理对脑缺血急性期的保护作用。方法:48只SD大鼠随机分为6组,每组8只。假手术组和模型对照组给予生理盐水,受试药给予葛根素注射液(21、42、84 mg/kg),阳性对照组给予灯盏细辛注射液(18 mg/kg)。尾静脉注射给药7天预防治疗后进行血栓栓塞性脑缺血手术。术后6 h行神经功能评分,采用核磁共振技术进行大鼠脑SE-T2WI以及弥散加权成像(DWI)序列扫描,测量脑病变范围;术后24 h评分后取脑进行TTC染色,测量脑梗死范围;取血清检测SOD、MDA。结果:葛根素42mg/kg预处理组大鼠缺血6 h和24 h行为障碍分别改善17.8%、33.3%;6 h脑病变范围和24 h梗死范围缩小29.4%、31.6%,血清MDA含量降低16.0%,SOD活力升高21.1%;葛根素84mg/kg较42mg/kg效果稍好,葛根素21mg/kg未见明显效果。结论:葛根素预处理能改善大鼠脑缺血急性期受损神经功能,缩小脑病变范围,具脑保护作用。更多还原

【Abstract】 Objective: To observe the effects of Puerarin preconditioning on the thromboembolic cerebral ischemia by Magnetic Resonance Imaging( MRI),TTC staining and pathological methods. Methods: Forty-eight SD rats were randomly divided into 4 groups( n = 8). Sham group and the model group were given saline,the reagent and positive control group were given puerarin injection( 21,42,84 mg / kg) and breviscapine injection( 4 ml / kg) for 7 days. Then the left middle cerebral artery of all rats was occluded by autologous blood clots. At 6h after MCAO,neurological deficit score and MRI including SE-T2 WI and DWI sequence scanning were measured. At 24 h after MCAO,the brain was cut for TTC staining and the serum was obtained to measure the oxidative stress after neurological deficit score. Results: At 6 h and 24 h,Puerarin 42 mg / kg preconditioning improved behavior disorder by 17. 8%( P > 0. 05) and 33. 3%( P < 0. 05); decreased brainlesion area and infarction scope by 29. 4%( P < 0. 05) and 31. 6%( P < 0. 05) respectively,and reduced serum MDA content by 16. 0%( P < 0. 05),increased SOD activity by 21. 1%( P < 0. 05),Puerarin 84 mg / kg had better effect than 42 mg / kg slightly,puerarin 21 mg / kg showed no effect. Conclusion: Puerarin pretreatment can improve the damaged neural function,decrease extent of disease,relieve the oxidative stress,with the cerebral protective effects for cerebral ischemia of rats.更多还原