【摘要】 目的观察睾丸支持细胞(Sertoli cells,SCs)和全反式维甲酸(all-trans retinoic acid,ATRA)对雌性骨髓干细胞(female bone marrow stem cells,FBMSCs)向FSHR+细胞分化的影响。方法将青年SD大鼠FBMSCs用全骨髓贴壁法分离,培养传代至第三代,用免疫细胞化学方法检测雌性骨髓干细胞、支持细胞FSHR的表达,Western blot检测分化细胞FSHR蛋白的表达情况。结果 western blot显示支持细胞共培养组和维甲酸组培养的雌性骨髓干细胞均有FSHR蛋白表达,对照组没有表达。免疫细胞化学显示,支持细胞共培养组和维甲酸组的雌性骨髓干细胞部分分化成为大而圆的FSHR阳性细胞。结论 FMSCs能向颗粒细胞样细胞分化的多能干细胞和定向分化为FSHR+细胞的能力。更多还原

【Abstract】 Objective:The aim is to investigate whether adult FBMSCs are able to differentiate into Follicle-stimulating hormone receptor positive(FSHR+)GCs like cells and Stra8(stimulated by retinoic acid gene 8,a specific expression gene in mammalian germ cell’s transition from mitosis to meiosis)expression oogonia in vitro by two different treatments:alltrans retinoic acid(ATRA)and indirect co-cultured with Sertoli cells(SCs).Methods:FBMSCs were separated from adult female SD rat bone marrow.FBMSCs the 3nd passage,Western blot and immunocytochemistry staining was utilized to examine the expression of FSHR in FBMSCs cultured with ATRA,indirect co-cultured with SCs and with SCs and ATRA,respectively. Results:The FBMSCs of the three treatments groups also expressed FSHR protein by Western Blotting. The FBMSCs of the control group did not express FSHR. Some FBMSCs were FSHR positive by immunocytochemistry following longer in culture(from day 5to day 7),the most of FSH+ cells gradually became larger.Some FBMSCs were FSHR positive by immunocytochemistry following longer in culture(from day 5 to day 7),the most of FSH+ cells gradually became larger. Conclusions:Adult FBMSCs contain pluripotent stem cells that could differentiate into gonadal somatic cells and differentiate into FSHR+ cells.更多还原