【摘要】 目的:建立高效液相色谱法测定碳酸钙D3咀嚼片中维生素D3含量的方法。方法:采用高效液相色谱法。以Diamonsil C18(250 mm×4.6mm,5μm)为色谱柱;流动相为甲醇;流速:1.0 ml·min-1;检测波长:264 nm。结果:维生素D3在4.8~38.4μg范围内。进样量与峰面积线性关系良好(r=1.0)。平均回收率为:97.82%(RSD=1.15%)。结论:方法简便、快速准确、灵敏度高,重现性好。可作为碳酸钙D3咀嚼片的质量控制方法。更多还原

【Abstract】 Objective: To establish a method for the determination of vitamin D3 in Calcium Carbonate and Vitamin D3 Chewable Tablets by HPLC. Methods: The HPLC system consisted of Diamonsil C18column( 250 mm × 4. 6 mm,5 μm),acetonitrile-water( 23 ∶ 77); flow rate at 1. 0ml·min-1and detected with UV at 264 nm. Results: The method had good linear relationship within the rage 4. 8 ~ 38. 4 μg of vitamin D3( r = 1. 0). The result( n = 6) of the recovery was 97. 82%( RSD = 1. 15%. Conclusion: The method is simple,fast,rapid,accurate and suitable for the determination of preparation.更多还原