【摘要】 研究典型中温嗜酸菌株Acidithiobacillus ferrooxidans ATCC 23270对两种不同形态单质硫(α-S8和μ-S)的利用和硫形态转化。A.ferrooxidans分别在α-S8或者μ-S中培养以适应能源底物。A.ferrooxidans在这两种单质硫中的生长和硫氧化行为的结果表明,A.ferrooxidans在α-S8中生长时比在μ-S中生长晚1 d进入对数期,但是A.ferrooxidans在α-S8中生长时在对数期生长较快且培养液具有较高的细胞浓度和较低的p H值。经过A.ferrooxidans的培养,这两种单质硫都被明显腐蚀和细胞修饰。此外,经过A.ferrooxidans的生长,无定型态的μ-S表面组分变为63.1%μ-S和36.9%α-S8,正交晶系的α-S8表面组分变为68.3%α-S8和31.7%μ-S;而无菌对照组μ-S和α-S8表面组分都没有变化,表明这两种不同形态的单质硫在A.ferrooxidans的作用下发生了相互转化。更多还原

【Abstract】 The utilization and speciation transformation of α-S8 and μ-S by the typical mesophilic acidophilic strain Acidithiobacillus ferrooxidans ATCC 23270 were investigated.A.ferrooxidans cells first acclimated to the energy source α-S8 or μ-S,respectively.The results of cell growth and sulfur oxidation behavior showed that the strain grown on α-S8 entered slowly(about 1 d later) into the exponential phase,while grew faster in the exponential phase and attained higher maximal cell density and lower p H value than that on μ-S.After bio-corrosion,both of the two sulfur samples were evidently eroded and modified by A.ferrooxidans cells.After growth of A.ferrooxidans,the surface composition of amorphous μ-S became 63.1% μ-S and 36.9% α-S8,and that of orthorhombic α-S8 became 68.3% α-S8 and 31.7% μ-S,while the surface compositions of α-S8 and μ-S in sterile experiment were not changed,indicating that these two elemental sulfur species can be interconverted by A.ferrooxidans.更多还原