【摘要】 目的:建立化生平胶囊的质量标准。方法:采用TLC法对黄芪、白花蛇舌草、丹参进行鉴别;用HPLC法测定黄芪甲苷的含量,色谱柱为Kromasil C18(250 mm×4.6 mm,5μm),流动相为乙腈-水(32∶68),流速:1.0 ml·min-1,检测波长:203nm柱温:25℃,进样量:20μl。结果:TLC色谱斑点清晰;黄芪甲苷在进样量为2.000~10.000μg范围内线性关系良好(r=0.999 6),平均回收率为100.8%,RSD为1.9%(n=6)。结论:该方法简便、准确、可靠,可用于化生平胶囊的质量控制。更多还原

【Abstract】 Objective: To establish the quality standard for Huashengping capsules. Methods: Milkvetch Root,Hedyotis diffusa Willd and Salvia miltiorrhiza were identified by TLC. The content of astragaloside A was detected by HPLC. The column was Kormasil C18(250 mm × 4. 6 mm,5 μm) and the flow rate was 1. 0 ml·min- 1. The mobile phases was a mixture of acetonitrile-water(32 ∶68). The detection wavelength was 203 nm. The column temperature was 25℃ and the sample size was 20 μl. Results: The TLC chromatography spots were clear. Astragaloside A was linear within the range of 2. 000-10. 000 μg( r = 0. 999 6) and the average recovery was 100. 8%( RSD = 1. 9%,n = 6). Conclusion: The method is simple,accurate and reliable,which can be used in the quality control of Huashengping capsules.更多还原