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芪蛭皱肺颗粒对LPS致炎大鼠AT-Ⅱ细胞SP-B表达的影响
Influences of Qizhi Zhoufei granules on express of pulmonary surfactant protein B in LPS-induced alveolar type Ⅱ cells
【摘要】 目的:通过观察芪蛭皱肺颗粒对脂多糖(LPS)致炎大鼠AT-II细胞SP-B表达变化的影响,探讨其在炎症条件下对SP-B的调控作用。方法:原代培养的AT-II细胞,分为空白组、模型组(脂多糖20μg/m L)、中药组(100μg/m L),用药组(均给予脂多糖20μg/m L,芪蛭皱肺颗粒高、中、低剂量分别为1000μg/m L、100μg/m L、10μg/m L);MTT法检测细胞活力;Q-PCR检测各组细胞SP-B m RNA表达水平。结果:与空白组对比,模型组细胞增殖异常,SP-B m RNA表达水平较低(P<0.05),与中药组无明显差别;与造模组比较,用药组细胞增殖减弱,与给药浓度相关,中剂量组SP-B m RNA表达水平显著提高(P<0.01)。结论:芪蛭皱肺颗粒能抑制脂多糖导致的AT-II细胞异常增殖,上调SP-B m RNA的低水平表达。
【Abstract】 Objective: To observe the effects of Qizhi Zhoufei granules on express of SP-B in LPS-indulesd inflammatory rats alveolar type II cells, and study its mechanism of regulatory effects of SP-B under inflammation. Methods: The primary cultured AT-II cells were divided into blank group, model group(LPS 20μg/m L),TCM group(100μg/m L), and medication group(LPS 20μg/m L, Qizhi Zhoufei Granules high-dose group with 1000μg/m L, Qizhi Zhoufei Granules medium-dose group with 100μg/m L, Qizhi Zhoufei Granules low-dose group with 10μg/m L); The viability of cells were detected by MTT assay. The expression level of SP-B m RNA of all groups were detected by Q-PCR assay. Results: Compared with the blank group, the model group’s cell in abnormal proliferation, the expression level of SP-B m RNA was lower(P<0.05), while its had no obvious difference in TCM group. Compared with the model group, cells in the medication group were weakened, it was positively correlated with concentration of Qizhi Zhoufei granules, the expression level of SP-B m RNA was remarkly increased(P<0.01). Conclusion: Qizhi Zhoufei granules could inhibit the abnormal proliferation of LPS-induced AT-II cells and enhance the low level expression of SP-B m RNA.
- 【文献出处】 中医临床研究 ,Clinical Journal of Chinese Medicine , 编辑部邮箱 ,2015年11期
- 【分类号】R285.5
- 【下载频次】61