【摘要】 目的评价联合应用微柱凝胶法和甘氨酸/EDTA放散试验进行诊断母婴血型不合新生儿溶血病(HDN)的临床价值。方法对355例疑为母婴血型不合HDN的黄疸患儿血标本联合应用微柱凝胶法和甘氨酸/EDTA放散试验检测。结果确诊为母婴血型不合HDN 277例(HDN的阳性检出率为78.03%),其中Rh-HDN 2例(0.72%),ABO-HDN 275例(99.28%)。ABO-HDN 275例中,母婴血型为O/A的占48.73%,O/B的占51.27%,B/AB和A/AB组合均为0例。在出生时间2 h≤n≤3 d、3 d<n≤5 d、5 d<n≤7 d、7 d<n≤13 d的黄疸儿中,HDN检出率分别为86.10%、73.02%、31.58%、14.29%。结论联合应用微柱凝胶法和甘氨酸/EDTA放散试验检测HDN,能够准确、及时地为临床诊治提供依据,值得推广。更多还原

【Abstract】 Objective To explore the clinical value of combined application of micro-gel method and glycine / EDTA diffuse method in the diagnosis of hemolytic disease of the newborn( HDN) caused by maternal-fetal blood type incompatibility. Methods Micro-gel method and glycine / EDTA diffuse method were jointly used to detect 355 blood samples from newborn with suspected HDN caused by maternal-fetal blood type incompatibility. Results HDN caused by maternal-fetal blood type incompatibility was diagnosed in 277 cases,the positive rate was 78. 03%,of which Rh-HDN was detected in 2 cases( 0. 72%),ABO-HDN in 275 cases( 99. 28%). In 275 cases of ABO-HDN,mother and baby were both blood type O / A accounted for 48. 73%,O / B accounted for 51. 27%,no B / AB and A /AB blood type. For these newborn with jaundice between 2 h≤n≤3 d,3 d < n ≤5 d,5 d < n ≤7 d,7 d < n≤13 d after birth. HDN positive detection rates were 86. 10%,73. 02%,31. 58%,14. 29%,respectively. Conclusion Joint application of micro-gel method and glycine / EDTA diffuse method in the detection of HDN,can accurately and timely provide the basis for clinical diagnosis and treatment.更多还原