【摘要】 目的观察活血滋肾汤对大鼠骨质疏松症模型血生化指标的影响,研究活血滋肾汤防治骨质疏松症的机制。方法采用8月龄健康雌性SD大鼠48只,按随机原则分为实验组、阳性组、模型组、正常组,每组各12只。正常组行假手术,其余3组行卵巢切除术,术后13周给药,持续12周后取血观测血清钙(Ca)、磷(P)、碱性磷酸酶(AKP)及骨钙素(BGP)、降钙素(CT)。结果 Ca、P水平在各组间相近(P>0.05);AKP测值实验组与阳性组、正常组相近(P>0.05),显著低于模型组(P<0.05);模型组BGP测值显著高于实验组、正常组和阳性组(P<0.05);实验组CT测值显著高于模型组(P<0.05)。结论活血滋肾汤不影响血清Ca、P水平,能抑制去势大鼠骨吸收作用,抑制高骨转换,调节成骨细胞活性,促进骨的生成,维持正常骨转换率,从而防治绝经后骨质疏松。更多还原

【Abstract】 Objective: To observe the effect of Huoxue Zishen Decoction on the blood biochemical index of osteoporosis rats model,and the study on the mechanism of Huoxue Zishen Decoction in prevention and treatment of osteoporosis. Methods: 48 8-month-old female SD rats,randomly divided into the experimental group,positive group,model group,and normal group,12 in each group. The normal group underwent sham operation,while other three groups were ovariectomized. Medicines were given 13 weeks later,lasting for 12 weeks. After that,blood was taken to observe the blood serum calcium(Ca),phosphorus(P),alkaline phosphatase(AKP) and osteocalcin(BGP),calcitonin(CT). Results: Ca and P did not differ among groups(P > 0.05);value of AKP measured in the experimental group and the positive group,the normal group had no difference(P > 0.05),significantly lower than that in the model group(P < 0.05);BGP of the model group was significantly higher than that of the experimental group,the normal group and the positive group(P < 0.05);CT of the experiment group was significantly higher than that of the model group(P < 0.05). Conclusion: Huoxue Zishen Decoction has no effect on serum calcium,and phosphorus level,but can inhibit bone resorption in ovariectomized rats,inhibit high bone turnover,adjust the activity of bone cells,promote bone formation,maintain normal bone turnover rate,and thus benefit the prevention and treatment of postmenopausal osteoporosis.更多还原