【摘要】 目的探讨郁金提取物对神经病理性疼痛模型(CCI)大鼠痛觉行为及脑源性神经营养因子(BDNF)表达的影响。方法建立CCI大鼠模型,将SD大鼠分为正常组、模型组、郁金提取物低、中、高剂量组,加巴喷汀组和溶剂组,对各组大鼠进行机械缩足阈值(PMWT)测定,用ELISA法测定大鼠血清中BDNF的表达。结果(1)与正常组相比,造模后大鼠PMWT显著下降(P<0.05),说明神经病理性模型造模成功;与溶剂组相比,郁金提取物高剂量组和加巴喷汀组显著上调CCI模型大鼠PMWT(P<0.05)。(2)造模后大鼠血液内BDNF表达均较正常组显著增加(P<0.05);与溶剂组相比,郁金提取物中、高剂量组和加巴喷汀组能显著降低血液中BDNF的表达(P<0.05)。郁金提取物高剂量组、加巴喷汀组和正常组BDNF表达无显著差异。结论郁金提取物可能通过降低CCI模型大鼠血清BDNF的表达而发挥抗神经病理性疼痛的作用。更多还原

【Abstract】 Objective: To investigate the effect of turmeric extraction on pain behavior and the BDNF expression in neuropathic pain model(CCI) rats. Methods: 36 SD rats were divided into normal group,model group,turmeric extract low,medium and high dose groups,gabapentin group and solvent group. Each rat was tested with PMWT,and BDNF expression in rats’ serums were detected by ELISA. Results:(1) Compared with the normal group,the thresholds of PMWT were significantly decreased after the establishment of CCI model(P < 0.05),which indicated the successful establishment of CCI rats model. Compared with the solvent group,PMWT thresholds were significantly upregulated in turmeric extract high dose group and gabapentin group(P < 0.05).(2)Compared with the normal group,BDNF expression were extraordinarily increased after the establishment of CCI model(P <0.05). Compared with the solvent group,the expressions of BDNF in serum in turmeric extract medium,high dose group and gabapentin group were significantly reduced(P < 0.05). Turmeric extraction high-dose group,gabapentin group and the normal group had no significant difference in BDNF expression. Conclusion: Turmeric extraction can attenuation neuropathic pain by reducing serum BDNF expression in CCI rat model.更多还原