【摘要】 目的用细胞模型探讨IL-1β诱导INS-1细胞凋亡,并观察寒温并用方对其保护作用。方法 MTT观察寒温并用方(1、0.5、0.25、0.125 g/L)分别作用24、48、72 h对INS-1细胞增殖的影响。寒温并用方(1、0.5、0.25、0.125 g/L)预处理细胞12 h,50 ng/L IL-1β继续处理细胞24 h,同时设空白对照组,IL-1β处理组。Annexin V-FITC流式细胞仪检测细胞凋亡情况。Western blotting检测各组caspase3,bcl-2 Bax蛋白表达。结果MTT显示寒温并用方以时间剂量依赖性促进INS-1细胞增殖。但1 g/L浓度在作用72 h后与其他浓度比较,显示明显的细胞抑制作用。Annexin V-FITC/PI结果显示IL-1β促进细胞凋亡。Western blotting结果表明IL-1β促进细胞凋亡蛋白合成,寒温并用方抑制其表达。结论寒温并用方能促进INS-1细胞增殖,并抑制IL-1β诱导INS-1的凋亡,可以用于炎症性胰岛细胞受损糖尿病的预防及治疗。更多还原

【Abstract】 Objective To investigate cellular model IL-1β-induced apoptosis in INS-1 cells,and observe the protective effect of Hanwenbingyong prescription. Methods The effects of Hanwenbingyong prescription( 1,0. 5,0. 25,0. 125 g / L) on proliferation of INS-1 cell were observed by MTT after acting 24,48 and 72 h. Hanwenbingyong prescription( 1,0. 5,0. 25,0. 125 g /L) pretreated cell for 12 h,and then 50 ng /L IL-1β-treated cells continue to 24 h. At the same time,made control group and IL-1β treatment group. Apoptosis was detected by V-FITC Annexin flow cytometry.Expression of Caspase3 and Bax Bcl-2 was detected by Western blotting. Results MTT showed Hanwenbingyong prescription promoted proliferation of INS-1 cells with a time and dose dependence. But the cells of 1g / L concentration were better than other groups after effected 72 h. Annexin V-FITC / PI showed that IL-1β promoted apoptosis. Western blotting showed that IL-1β promoted apoptosis protein synthesis,Hanwenbingyong prescription inhibited expression of IL-1β. Conclusion Hanwenbingyong prescription can promote proliferation of INS-1 cells,and inhibit the apoptosis of INS-1 induced by IL-1β,which can be used in prevention and treatment of inflammatory islet cell damage.更多还原