【摘要】 目的:观察柴苓汤对慢性环孢素A肾病大鼠单核细胞趋化蛋白-1(monocyte chemoattractant protein-1,MCP-1)及肿瘤坏死因子-a(tumour necrosis factor-a,TNF-a)的影响,探讨炎症反应在环孢素A肾病中的作用及柴苓汤的调控机制。方法:采用经口灌服环孢素A[30 mg/(kg·d)]的方法复制慢性环孢素A肾病大鼠模型,同时给予柴苓汤[3 g/(kg·d)]及缬沙坦[10 mg/(kg·d)]治疗共28 d,摘取肾脏观察肾脏病理变化。RT-PCR、免疫组化方法检测大鼠肾脏MCP-1、TNF-a蛋白及mRNA的表达。结果:病理显示模型组大鼠肾脏大量炎性细胞浸润,肾小管间质胶原成分明显增多,与对照组比较,MCP-I、TNF-a蛋白及mRNA表达显著增强。经柴苓汤及缬沙坦治疗,大鼠肾脏炎性细胞浸润及胶原沉积均减轻,MCP-I及TNF-a高表达被显著下调。结论:柴苓汤可减轻炎症损伤,减少ECM沉积,从而延缓慢性Cs A肾病纤维化进程。更多还原

【Abstract】 Objective: To observe the effect of Chailing Decoction( CLD) on monocyte chemoattractant protein-1( MCP-1) and tumour necrosis factor-α( TNF-α) in rats with chronic cyclosporine A nephropathy( CCN) for investigating the function of inflammation and regulatory mechanism of CLD. Methods: The CCN rat models were prepared using oral administration of cyclosporine A( 30 mg·kg- 1·d- 1). Meanwhile,they were treated with CLD( 3 g·kg- 1·d- 1) and valsartan( 10 m g·kg- 1·d- 1) by gastrogavage for 28 days respectively. The kidneys were harvested on the 29 th day and observed the pathology changes. The protein and gene expressions of MCP-I and TNF-a were observed using RT-PCR and immunohistochemical assay. Results: Renal pathology showed the inflammatory cell infiltration and collagen deposition in the renal interstitial area of Cs A group was significantly increased. Compared with the control group,the protein and mRNA expressions of MCP-I and TNF-a of Cs A group were significantly enhanced,and the high levels were down-regulated after treatment. Conclusions: CLD could retard the progress of CCN renal interstitial fibrosis by alleviating inflammation and reducing the deposition of ECM.更多还原