【摘要】 目的:检测不同时期支原体肺炎(MPP)患儿外周血Treg细胞、Th17细胞及其相关细胞因子TGF-β、IL-10、IL-17和IL-6水平,观察其变化规律,探讨MPP急性期免疫失衡发病机制。方法:40例MPP患儿及20例非MP感染性支气管肺炎在急性期(发病<2周)及病程4周时空腹抽取外周静脉血5 m L,使用流式细胞仪检测Treg细胞、Th17细胞的百分率,ELISA方法检测TGF-β、IL-10、IL-17和IL-6细胞因子水平,同时与20例健康体检儿进行比较。结果:急性期MPP组Treg细胞百分率低于正常对照组(P<0.05);病程4周时,MPP组Treg细胞百分率明显提高,MPP组TGF-β、IL-10水平低于非MP感染组及正常对照组(P<0.05)。急性期及病程4周时,MPP组患儿Th17细胞百分率及IL-17、IL-6水平均高于非MP感染组和正常对照组患儿(P<0.05)。结论:儿童MPP感染与免疫调节紊乱密切相关,急性期存在明显的免疫失衡,Treg/Th17细胞失衡及其相关效应因子的表达在儿童MPP感染起到重要的作用。更多还原

【Abstract】 Objective: To observe the percentages of Treg cells and Th17 cells, the levels of IL-10,TGF-β,IL-17 and IL-6 in peripheral blood of children with mycoplasma pneumoniae pneumonia and explore the role of them in pathogenesis of MPP. Method: 40 children with MPP,20 children with non- MPP and 20 healthy children were enrolled.The percentages of Treg cells and Th17 cells in peripheral blood were detected by flow cytometer（FCM）,and the levels of IL-10,TGF-β,IL-17 and IL-6 in plasma were determined by ELISA, at the same time, compared with 20 cases healthy children. Result: In the acute stag, The percentage of Treg cells in children with MPP were significantly lower than healthy infants（P<0.05）. The percentage of Treg cells in the 4^th week were significantly higher than those in the acute stag, the levels of IL-10 and TGF-βin children with MPP were significantly lower than those in children with non- MPP and healthy infants in the acute stag and the 4^th week（P<0.05）. The percentage of Th17 cells and the levels of IL-17 and IL-6 in children with MPP were significantly higher than those in children with non- MPP and healthy infants in the acute stag and the 4^th week（P<0.05）,while the percentage of Th17 cells and the levels of IL-17 and IL-6 in children with non- MPP were significantly low in the 4^th week, it was not significant difference between the children with MPP and the children with non-MPP. Conclusion:There is markedly mimunoresponsiveness unbalance in acute phase of MPP. Treg cell,Th17 cells, IL-10, TGF-β, IL-17 and IL-6 may be involved in pathogenesis of MPP.更多还原