【摘要】 目的:检测胎儿生长受限胎盘及正常足月胎儿胎盘中激活素受体的表达,分析激活素受体表达与胎儿生长受限的关系。方法:选取16例足月胎儿生长受限胎盘作为观察组和正常足月胎盘组织15例作为对照组,胎盘组织采用HE染色法分析,胎盘组织中激活素受体的表达部位采用免疫组织化学法分析,并进行半定量分析,激活素受体在胎盘m RNA水平的表达采用PCR法分析。结果:两组激活素受体ⅠA、ⅠB表达差异不明显(P>0.05),观察组ⅡA、ⅡB在(++),(+++)的百分比均高于对照组(P<0.05),观察组ⅡA、ⅡB在(-)的百分比显著低于对照组(P<0.05);观察组胎盘组织激活素受体ⅠA、ⅠB基因表达与对照组比较差异无统计学意义(P>0.05),观察组胎盘组织ⅡA、ⅡB表达明显高于对照组(P<0.05)。结论:胎儿生长受限患者胎盘激活素水平明显要高于正常胎盘,激活素紊乱会导致胎儿生长受限。更多还原

【Abstract】 Objective:To detect normal fetal restricted normal term placenta and fetal placental activin receptor expression,analyze activin receptor expression and fetal growth retardation.Method:16 cases of fetal growth restriction at term placenta were selected as the observation group and 15 cases of normal term placenta were selected as a control group,placental tissue by HE staining analysis,site of placental tissue expression of activin receptor by immunohistochemistry method analyzed and semi-quantitative analysis,activin receptor m RNA expression level in the placenta by PCR analyzed.Result:The activin receptor ⅠA,ⅠB expression was not significantly different of the two groups(P>0.05),the observation group type ⅡA and ⅡB in(++),(+++) percentage were higher than the control group(P<0.05);the observation group type ⅡA and ⅡB in(-) the percentage of significantly lower than control group(P<0.05).Placental tissue of patients in the observation group activin receptor ⅠA,ⅠB and gene expression in the control group no significant difference(P>0.05),placenta observation group ⅡA,ⅡB expression was significantly higher(P<0.05).Conclusion:Fetal growth restriction placental activin levels were significantly higher than normal placenta,activin disorder can lead to fetal growth restriction.更多还原