Objective: To investigate the anticoagulant effect of TY602-1 and its mechanism. Methods: In vitro and in vivo studies were carried out to evaluate the anticoagulation activity,such as the enzyme assay in vitro,measurement of mouse plasma prothrombin time( PT),activated partial thromboplastin time( APTT) and thrombin time( TT),arteriovenous shunt model in rats,and rat venous stasis model. Results: TY602-1 could inhibit the activity of the coagulation factor FⅩ a. Intragastric administration of 1. 25,2. 5,5,...