【摘要】 目的:运用蛋白质组学的研究方法,分析膈下逐瘀汤加减方(GXZY)含药血清作用人肝癌细胞SMMC-7721的差异蛋白质表达情况,以期寻找GXZY抗肿瘤作用靶点。方法:将人肝癌细胞SMMC-7721传代培养后,随机分为GXZY血清组以及空白对照组,应用双向凝胶电泳、MALDI-TOF-MS鉴定分析差异蛋白质表达情况。结果:GXZY血清组和空白对照组的蛋白电泳图谱上各有33个蛋白质点高表达,经质谱分析鉴定出6个蛋白质点,GXZY血清组有5个蛋白质点AHCY、PGAM1、STMN1、HSP90AB1、VAT-1表达下调,1个蛋白质点TPM4表达上调。结论:GXZY含药血清处理的人肝癌细胞SMMC-7721蛋白质质谱表达有差异,这些差异蛋白质可能是GXZY治疗的作用靶点。更多还原

【Abstract】 Objective: To analyze the differential expression protein of human hepatic carcinoma cell line SMMC-7721 under the Gexia Zhuyu decoction( GXZY) pharmacological serum,in order to find the anti-tumor target of GXZY. Methods: Subcultured human hepatic carcinoma cell line SMMC-7721 were randomly divided into GXZY pharmacological serum groups and blank drug serum group,using two-dimensional gel electrophoresis,MALDI-TOF-MS to analyze the differential expression protein. Results: High expression of 33 protein spots was found in each of the groups,6 proteins were identified by mass spectrometry analysis. Expression of 5 protein spots in GXZY pharmacological serum group decreased( AHCY,PGAM1,STMN1,HSP90AB1,VAT1) and 1 increased( TPM4). Conclusion: There are differential expression proteins of human hepatic carcinoma cell line SMMC-7721 in GXZY pharmacological serum,these differential expression proteins may be the therapeutic targets of GXZY.更多还原