【摘要】 目的探讨依达拉奉干预大鼠肢体缺血再灌注损伤后对Na+-K+-ATP酶和Ca2+-ATP酶活性的影响及超微结构的改变,为治疗肢体缺血再灌注损伤提供新的方法。方法制作大鼠肢体缺血再灌注损伤模型,依达拉奉灌注组(ME组)于再灌注前5 min经左股静脉注射依达拉奉,模型组(M组)及正常组(N组)则给予等量的生理盐水。于再灌注24 h时,取每组大鼠右胫前肌,用光镜及电镜观察其形态学变化,并检测Na+-K+-ATP、Ca2+-ATP酶活力。采用方差分析和LSD法进行统计学分析。结果 (1)光镜结果显示:ME组比M组肌纤维形态较整齐,形态较整齐,水肿程度及炎细胞浸润均减轻;(2)电镜结果显示:ME组与M组比较,M线、Z线较清晰,线粒体肿胀减轻,线粒体数量及嵴增多;(3)Na+-K+-ATP酶活力显示,N组(13.24±2.14)U/mgprot酶活力最高,ME组[(10.15±1.79)U/mgprot]和M组[(5.19±0.58)U/mgprot]酶活力逐渐减低,差异有统计学意义(F=60.817,P<0.001),组间差异均有统计学意义(P<0.05);(4)Ca2+-ATP酶活力显示:N组[(2.71±1.38)U/mgprot]酶活力高于ME组[(1.89±0.37)U/mgprot],以M组[(0.32±0.27)U/mgprot]酶活力最低,差异有统计学意义(F=21.056,P<0.001),组间差异均有统计学意义(P<0.05)。结论依达拉奉可以减轻大鼠肢体缺血再灌注区域线粒体的损伤,相应地提高Na+-K+-ATP酶及Ca2+-ATP酶的活力,从而减轻肢体缺血与再灌注损伤,为临床缺血与再灌注损伤的预防及治疗提供新的方法。更多还原

【Abstract】 Objective The study explored the effect of edaravone on Na+-K+-ATPase, Ca2+-ATPase activity and ultrastructure changes in rat limb ischemia reperfusion injury in order to provide a new treatment for limb ischemia reperfusion injury. Methods Rats were randomized into three groups after limb ischemia reperfusion injury was induced. Group ME(edaravone reperfusion group)was given edaravone before reperfusion 5 min via the left femoral vein. Group M(model group)and Group N(normal group)were given an equal volume of physiological saline. The right anterior tibial muscles of each group were removed after 24 h of reperfusion, the morphological changes were observed under light microscope and electron microscope, and the activity of Na+-K+-ATP and Ca2+-ATP enzyme were detected.Statistical analysis was performed by analysis of variance and LSD. Results(1)Light microscope results: compared with Group M, the muscle fibers were well-organized and the degree of edema and inflammatory cell infiltration of muscle were lower in Group ME.(2)Electron microscope results: compared with Group M, the muscle M line and Z line were clearer in Group ME, with decreased mitochondrial swelling and increased numbers of mitochondria and mitochondrial crista.(3)Na+-K+-ATPase activity: The enzyme activity in Group N(13.24 ± 2.14)was highest, followed by Group ME[(10.15 ± 1.79)U/mgprot] and Group M[(5.19 ± 0.58)U/mgprot], the difference was statistically significant(F = 60.817, P < 0.001), LSD differences were statistically significant between groups(P < 0.05).(4)Ca2+-ATPase activity: The enzyme activity in Group N[(2.71 ± 1.38)U/mgprot] was higher than that in Group ME[(1.89 ± 0.37)U/mgprot], the enzyme activity in Group M[(0.32 ± 0.27) U/mgprot] was lowest, single factor variance analysis showed the difference was statistically significant(F = 21.056, P < 0.001). Conclusion Edaravone can reduce rat limb ischemia reperfusion injury of mitochondria, increase Na+-K+-ATPase and Ca2+-ATPase activity. The potential clinical application deserves further study.更多还原