【Objective】To analyze the chromosome aneuploidy of low grade embryos with fluorescence in situ hybridization(FISH) and to evaluate whether low grade embryo transplantation is a safe choice. 【Methods】D3 low grade embryos were collected from IVF/ICSI cycles and fixed. Then the nuclei were detected using eight different chromosome probes in two rounds of FISH. 【Results】Totally 178 blastomeres were obtained from 40 D3 low grade embryos in which 170 nuclei were fixed successfully. The fix rate was 95.5%(170/178)...