以小麦光温敏核雄性不育系BS20×Fu3双单倍体(DH)群体的289个系为材料,从1112对SSR和EST-SSR引物中筛选出多态性引物243对,利用其中128个SSR和6个EST-SSR标记构建遗传连锁图谱,该图谱覆盖长度为2749.2 c M,分布在小麦的19个连锁群(除4D、6A),不同连锁群标记数为2~15个,长度在15.3~244.4 c M之间,平均长度为144.7 c M,标记之间平均遗传距离为17.4 c M。同时构建3个DNA池(包括恢复池、北京不育池和阜阳不育池),用分离群体分组分析法(BSA)对育性进行分析,筛选出的多态性引物为Wmc264、Wmc73、Xgwm350,分布在3A、5B、2A/7D染色体上。同时用混合线性复合区间作图法(MCIM)对育性进行QTL分析,当F>7.5时,检测到6个主效QTL,用复合区间作图法(CIM)对育性进行QTL分析,当LOD值>2.5时,共检测到13个主效QTL,两种方法检测到一致的QTL有3个,分别为1BL的Wmc365-cfa2129、2BS的Wmc602-Xgwm148和3AL的Wmc264a-cfa2262区间的QTL。综合BSA和QTL的结果...
【英文摘要】
The genetic linkage map of wheat was constructed with DH population consisting of 289 individuals from a cross between BS20 and Fu3. 1112 SSR and EST-SSR primers were used to detect the polymorphisms between the parents. Applying QGA_CN software to carry out linkage analysis,the genetic map covered 19 chromosomes except 4D and 6A which included 128 SSR and 6 EST-SSR markers. The linkage map covered 2749. 2 c M and the average distance was 17. 4 c M. All 19 linkage groups consisted of 2-15 markers,with the c...